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Horse conformation is sometimes described as a complex or polygenic trait.This means that it is affected by both many genes and the environment.This page deals with horse reproduction in a genetic sense, rather than from the point of view of horse breeding practicalities.There is just enough here about the process of meiosis and fertilisation to understand how genes are inherited during horse reproduction, plus a little on how these genes are then passed on to the cells of the body.

Homozygous and heterozygous tobiano horses are indistinguishable on the basis of phenotype (external appearance) alone.

Even then there may be a range of possible foal colours for a cross between any given pair of horses.

In cases where a "brown" colouration is due to a dilution of black then crossing brown horses together would result in black, brown and chestnut foals, and other dilute types depending on whether the dilution gene(s) is/are dominant or recessive, and whether red pigment is affected as well as black (perhaps to make chestnut into sorrel).

However the presence of only tobiano foals among a tobiano horses offspring doesn’t prove that the horse is true-breeding tobiano, especially if only a few foals have been produced.

No direct DNA test for the tobiano gene is available yet, but “tobiano zygosity analysis” can be used to identify tobiano horses likely to be homozygous for the tobiano gene.

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